Submitted: 21 May 2018
Accepted: 18 Jun 2018
First published online: 30 Sep 2018
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Int J Basic Sci Med. 2018;3(3):127-132.
doi: 10.15171/ijbsm.2018.23
  Abstract View: 178
  PDF Download: 184

Original article

Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats

Parastoo Rahdar 1, Homayoun Khazali 1 *

1 Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran


Introduction: Gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologue RF amide-related peptide (RFRP) are known to inhibit the secretion of gonadotropins. In addition, substance P (SP), a member of tachykinin’s family, can increase the firing rate of kisspeptin/ neurokinin B/dynorphin (KNDy) neurons and provoke the secretion of gonadotropins. In this experimental study, we investigated the effects of co-administration of RFRP-3 and SP on the expression of KISS-1 and GnRH genes, as gonadotropin regulator genes, in male rats.

Methods: Forty-two mature Wistar rats were randomly allocated into 7 groups (n=6 in each group). Animals in each group intracerebroventricularly received either saline+DMSO, SP (1nmol), RFRP-3 (5 nmol), SP (1nmol) + RFRP-3 (5 nmol), SP (1 nmol) + RF9 (RFRP-3 receptor antagonist, 10 nmol), SP (1 nmol) + P234 (kisspeptin receptor antagonist, 1 nmol) + RFRP-3 (5 nmol), or SP (1 nmol) + CP-96,345 (SP receptor antagonist, 5 nmol) + RFRP-3 (5 nmol). Two hours after injections, hypothalamic samples were collected to evaluate the expression of target genes by real-time PCR.

Results: Injections in SP and SP + RF9 groups increased the expression of both GnRH and KISS-1 genes (P<0.05). Injections in RFRP-3 and SP + RFRP-3 + CP-96,345 groups significantly decreased the expression of GnRH and KISS-1 genes (P<0.05). However, injections of SP + RFRP-3 and SP + RFRP-3 + P234 did not significantly change the expression of GnRH and KISS1 genes.

Conclusion: The results of our study indicated that SP antagonized the effects of RFRP-3 in the expression of hypothalamic KISS-1 and GnRH genes.

Please cite this article as follows: Rahdar P, Khazali H. Central injection of substance P antagonizes the RF amide-related peptide-3 impacts on hypothalamic KISS-1 and GnRH gene expressions in male waster rats. Int J Basic Sci Med. 2018;3(3):127- 132. doi:10.15171/ ijbms.2018.23.
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