Submitted: 03 Dec 2018
Accepted: 23 Feb 2019
First published online: 30 Jun 2020
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Int J Basic Sci Med. 2020;5(2):43-47.
doi: 10.34172/ijbsm.2020.09
  Abstract View: 28
  PDF Download: 30

Original article

The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection

Parisa Shahbazi 1, Mohammad Jahantigh 2 * , Saeed Salari 3, Salehe Danesh 4

1 Graduated Student of Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
3 Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
4 Zabol University of Medical Sciences, Zabol, Iran
*Correspondence to Mohammad Jahantigh, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran. Tel: +989151422235 Email: Email: mjahantig@yahoo.com

Abstract

Introduction: The production of β-lactamase in bacteria, especially in Escherichia coli as a prevalent opportunistic bacterium, has caused many problems in patient treatment. β-lactamases are encoded by extended-spectrum β-lactamase (ESBL) genes such as blaTEM and blaCTX-M. We aimed to assess the prevalence and antibiotic sensitivity of β-lactamases encoded by blaCTX-M and blaTEM in E. coli isolated from patients suffering from urinary tract infections (UTIs).

Methods: Escherichia coli strains were isolated from the patients’ urine culture presented to medical diagnostic laboratories in Zabol, Iran. The agar disc-diffusion test was performed on Müller-Hinton agar to investigate the antibiotic resistance of these isolates using eight antimicrobial paper discs including gentamicin, tetracycline, co-trimoxazole, norfloxacin, cefuroxime, ampicillin, neomycin, and amoxicillin. A conventional polymerase chain reaction (PCR) was used to detect blaCTX-M and blaTEM.

Results: The frequencies of resistance to cefuroxime, norfloxacin, co-trimoxazole, neomycin, amoxicillin, tetracycline, gentamicin, and ampicillin were found to be 45 (90%), 15 (30%), 33 (66%), 33 (66%), 44 (88%), 34 (68%), 4 (8%), and 50 (100%), respectively. Moreover, the prevalence of blaCTX-M was 25 (50%) while that of blaTEM was 16 (32%).

Conclusion: Based on the results, gentamicin and norfloxacin can be recommended as effective antibacterials for treating UTI caused by E. coli in the study population. Moreover, the frequency of resistant genes including blaCTX-M and blaTEM was high in the isolated E. coli. Effective control systems including appropriate treatments for ESBL-producing strains are therefore required for humans and food animals.

Please cite this article as follows: Shahbazi P, Jahantigh M, Salari S, Danesh S.The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection. Int J Basic Sci Med. 2020;5(2):43- 47. doi:10.34172/ ijbms.2020.09.
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