Abstract
Introduction: MiRNA expression alterations are closely related to growth, proliferation, and cell
development. In addition, miR-187 has been found to regulate cellular proliferation. Further,
spermatogenesis is a highly complicated process which is regulated through several genes
targeted by miRNAs. However, no study has completely addressed the etiology of spermatogenic
impairments. Accordingly, the present study examined the expression pattern of Homo sapiens
(human) microRNA (hsa-mir-187) in the testicular biopsies of infertile men with non-obstructive
azoospermia (NOA) and Sertoli cell-only syndrome (SCOS) subtype in comparison with those
with obstructive azoospermia (OA).
Methods: Quantitative reverse‐transcription polymerase chain reaction analysis was performed
to assess the expression level of hsa-mir-187 using the ΔΔCt method.
Results: Our data indicated that hsa-mir-187 was significantly up-regulated in the testis of the
SCOS group (P˂ 0.05) in comparison with the OA group.
Conclusion: In general, the findings suggest that the differential expression of hsa-mir-187 is
linked with male infertility and shed more light on molecular defects that underlie spermatogenic
impairments.