Submitted: 27 Apr 2019
Accepted: 26 Jun 2019
First published online: 30 Sep 2019
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Int J Basic Sci Med. 2019;4(3):113-118.
doi: 10.15171/ijbsm.2019.21
  Abstract View: 71
  PDF Download: 96

Original article

Evaluation of Anti-cancer and Pro-apoptotic Activities of Iranian Green Tea Extract Against A549,PC3, and MCF-7 Cancer Cell Lines

Fatemeh Safari 1 * , Masomeh Rabieepor 2, Fatemeh Jamalomidi 1, Zahra Baghaeifar 2, Leila Khodaei 3

1 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
2 Department of Biology, Payame Noor University (PNU), Tehran, Iran
3 Department of Agriculture, Payame Noor University (PNU), Tehran, Iran
*Correspondence to Fatemeh Safari, Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran. E-mail: Email: safarif2003@gmail.com

Abstract

Introduction: Green tea contains active polyphenols including catechins. The goal of the current study was to evaluate anti-cancer effects of Iranian green tea extract (IGTE) on 3 human cancer cell lines including A549, PC3, and MCF-7.

Methods: First, Camellia sinensis was obtained from Lahijan, a city in the north of Iran and then IGTE was prepared. Next, catechins of IGTE were determined using high-performance liquid chromatography (HPLC). Finally, the cell viability of different cancer cells was evaluated by treatment with IGTE at concentration between 100 and 1000 µg/mL for 72 hours using MTT assay. Cell death of treated cancer cells was assessed by DAPI staining and RT-PCR method.

Results: Our results demonstrated the potential anti-tumor activity of IGTE on MCF-7 cells (IC50= 400 µM), A549 cells (IC50= 500 µM), and PC3 cells (IC50= 600 µM), respectively. Chromatin damages within the nucleus of the treated cancer cells were shown. In addition, we found that IGTE induced apoptosis by up-regulation of Bax (a pro-apoptotic protein) and down-regulation of Bcl2 (an anti-apoptotic protein).

Conclusion: Herein, we showed that IGTE is a potent natural product with anti-tumor activity on breast, lung, and prostate cancer cells. The efficacy of current therapies against cancer is limited by a range of adverse effects, toxicity, and drug resistance; therefore, new therapeutic strategies and more effective agents, particularly with natural origin, are desired and green tea may be a potent candidate in the field of cancer therapy

Please cite this article as follows: Safari F, Rabieepor M, Jamalomidi F, Baghaeifar Z, Khodaei L. Evaluation of anti-cancer and pro-apoptotic activities of Iranian green tea extract against A549, PC3, and MCF-7 cancer cell lines. Int J Basic Sci Med. 2019;4(3):113- 118. doi:10.15171/ ijbms.2019.21.
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