Submitted: 17 Jan 2021
Revised: 29 Apr 2021
Accepted: 24 May 2021
First published online: 04 Jun 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

Int J Basic Sci Med. 2021;6(2):51-56.
doi: 10.34172/ijbsm.2021.09
  Abstract View: 132
  PDF Download: 138

Original Article

Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion

Mohammad Ashna 1 ORCiD, Ali Es-haghi 1 * ORCiD, Mojgan Soltani 1

1 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Abstract

Introduction: Cancer can be defined as an illness of reformed gene expression. There are numerous agents affecting gene expression and altering cellular activities. Nanotechnology has offered the possibility of modulating tumor suppressor genes’ expression, improving kinetics of gene-targeted therapeutics, and simplifying drug delivery to tumors and across bio-complexes.Methods: Gene expressions of caspase3, 8 and 9, Bax and Bcl2 were assessed after RNA extraction and cDNA synthesis by real-time polymerase chain reaction (PCR) in an ovarian cancerous cell line (A2780). Real-time PCR was employed to determine the change fold of apoptotic genes in the cells exposed to the biosynthesized CeO2-NPs (cerium oxide nanoparticles) at the dose of 0, 7, 9, and 11 μg/mL after 24 hours of incubation. Results: Our findings displayed a significant increase in the antioxidant genes’ expression in the A2870 cells. The results exhibited that the biosynthesized CeO2-NPs could enhance the Bax/Bcl2 ratio in a dose-dependent way. Also, the expression of caspase3, 8, and 9 up-regulated significantly under the dose of 11 μg/mL.Conclusion: Considering the effects of the bio-fabricated CeO2-NPs on the expression of apoptotic genes in ovarian cancerous cell lines, these nanoparticles (NPs) may be employed in pharmacology to develop new anti-cancer medications.
First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 132

Your browser does not support the canvas element.


PDF Downloaded: 138

Your browser does not support the canvas element.