Int J Basic Sci Med. 2016;1: 25-28.
doi: 10.15171/ijbsm.2016.06
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Original article

Evaluation of the Antimicrobial Activity of Silver Nanoparticles on Antibiotic-Resistant Pseudomonas aeruginosa

Aliakbar Nasiri 1, Roghayeh Afsar Gharebagh 2, Seyed Ali Nojoumi 3, Majidreza Akbarizadeh 4, Sharareh Harirchi 5, Masoud Arefnezhad 6, Shahla Sahraei 7, Mehran Hesaraki 4, Mahdi Afshari 6, Fereshteh Javadian 6*, Maryam Sheykhzade Asadi 8, Zahra Shahi 8, Aliyeh Sargazi 9

1 Assistant Professor of Anesthesiology, Department of Anesthesiology, Zabol University of Medical Sciences, Zabol, Iran
2 Assistant Professor of Cardiology, Department of Cardiology, Urmia University of Medical Sciences, Urmia, Iran
3 Assistant Professor of Microbiology, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
4 Assistant Professor of Pediatrics, Department of Pediatrics, Zabol University of Medical Sciences, Zabol, Iran
5 PhD Student of Microbiology, Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
6 Zabol University of Medical Sciences, Zabol, Iran
7 Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran
8 Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran
9 Student Research Committee, Zabol University of Medical Sciences, Zabol, Iran
*Corresponding Author: *Correspondence to Fereshteh Javadian , Email: fereshteh.javadian@yahoo.com


Background: Antimicrobial resistance is one of the major characteristics of infectious agents. Silver nanoparticles (AgNPs) have been introduced as novel antibacterial agents in accordance with the traditional treatments. Our purpose of this study was to evaluate the antimicrobial activity of AgNPs on the Pseudomonas aeruginosa (P. aeruginosa) that are resistant to antibiotics. 

Methods: During a cross-sectional study, we tried to evaluate 20 strains of P. aeruginosa isolated from the urine cultures of patients admitted to the hospital due to urinary tract infections. The AgNPs were commercially purchased. The minimum inhibitory concentration (MIC) of AgNPs in different concentrations was determined by the dilution in wells on bacteria. The antibiotic susceptibility pattern of P. aeruginosa was evaluated by the Kirby-Bauer disk diffusion standard. 

Results: Current study indicated that P. aeruginosa were resistant to four types of agents including ampicillin (85%), nitrofurantoin (65%), nalidixic acid (65%), and ciprofloxacin (15%) and result of nanosilver indicated that the most MIC was 100 ppm concentration, and six strains of P. aeruginosa were inhibited by it. 

Conclusion: Our study presented a new type of silver nanoparticle and indicated that they can be embedded in bone cement to prevent infections once synthetic conditions are tailored for such applications. 

Keywords: Nanosilver, Antimicrobial activity, Pseudomonas aeruginosa, Minimum inhibitory concentrations (MIC), Antibiotic-resistant

Please cite this article as follows: Nasiri A, Afsar Gharebagh R, Nojoumi SA, et al. Evaluation of the antimicrobial activity of silver nanoparticles on antibiotic-resistant Pseudomonas aeruginosa. Int J Basic Sci Med. 2016;1(1):25-28. doi:10.15171/ijbms.2016.06.
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