Aliakbar Nasiri 1
, Roghayeh Afsar Gharebagh 2
, Seyed Ali Nojoumi 3
, Majidreza Akbarizadeh 4
, Sharareh Harirchi 5
, Masoud Arefnezhad 6
, Shahla Sahraei 7
, Mehran Hesaraki 4
, Mahdi Afshari 6
, Fereshteh Javadian 6*
, Maryam Sheykhzade Asadi 8
, Zahra Shahi 8
, Aliyeh Sargazi 91
Assistant Professor of Anesthesiology, Department of Anesthesiology, Zabol University of Medical Sciences, Zabol, Iran2
Assistant Professor of Cardiology, Department of Cardiology, Urmia University of Medical Sciences, Urmia, Iran3
Assistant Professor of Microbiology, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran4
Assistant Professor of Pediatrics, Department of Pediatrics, Zabol University of Medical Sciences, Zabol, Iran5
PhD Student of Microbiology, Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran6
Zabol University of Medical Sciences, Zabol, Iran7
Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran8
Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran9
Student Research Committee, Zabol University of Medical Sciences, Zabol, Iran
Background: Antimicrobial resistance is one of the major characteristics of infectious agents. Silver nanoparticles (AgNPs) have been introduced as novel antibacterial agents in accordance with the traditional treatments. Our purpose of this study was to evaluate the antimicrobial activity of AgNPs on the Pseudomonas aeruginosa (P. aeruginosa) that are resistant to antibiotics.
Methods: During a cross-sectional study, we tried to evaluate 20 strains of P. aeruginosa isolated from the urine cultures of patients admitted to the hospital due to urinary tract infections. The AgNPs were commercially purchased. The minimum inhibitory concentration (MIC) of AgNPs in different concentrations was determined by the dilution in wells on bacteria. The antibiotic susceptibility pattern of P. aeruginosa was evaluated by the Kirby-Bauer disk diffusion standard.
Results: Current study indicated that P. aeruginosa were resistant to four types of agents including ampicillin (85%), nitrofurantoin (65%), nalidixic acid (65%), and ciprofloxacin (15%) and result of nanosilver indicated that the most MIC was 100 ppm concentration, and six strains of P. aeruginosa were inhibited by it.
Conclusion: Our study presented a new type of silver nanoparticle and indicated that they can be embedded in bone cement to prevent infections once synthetic conditions are tailored for such applications.
Keywords: Nanosilver, Antimicrobial activity, Pseudomonas aeruginosa, Minimum inhibitory concentrations (MIC), Antibiotic-resistant