Introduction: Considering the usefulness of metal oxide nanoparticles in biology and
biomedicine, iron oxide nanoparticles were biosynthesized using bioresource engineering to
evaluate its antibacterial activity against Escherichia coli.
Methods: Macrodilution method was used for calculating the lowest concentration which
prevented the growth of bacteria (minimum inhibitory concentration [MIC]), and the lowest
concentration that destroyed all bacterial cells (minimum bactericidal concentration [MBC]).
Results: The lowest concentration of iron oxide nanoparticles that inhibited the growth of E. coli
(MIC) was recorded at 250 µg/mL. On the other hand, the MBC of iron oxide nanoparticles was
calculated at 500 µg/mL.
Conclusion: Iron oxide nanoparticles were produced by a green and eco-friendly, simple and
inexpensive method. The results showed the inhibitory effect of iron oxide nanoparticles on E.
coli at 250 µg/mL. This may suggest using these nanoparticles as potential antibacterial agents.