Logo-ijbsm
Submitted: 15 Jan 2024
Accepted: 18 Feb 2024
ePublished: 08 Mar 2024
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Int J Basic Sci Med. 2024;9: 1-5.
doi: 10.34172/ijbsm.46653
  Abstract View: 263
  PDF Download: 56

Original Article

Unveiling Efflux Pump-Mediated Antibiotic Resistance in Klebsiella pneumoniae Clinical Isolates: A Call for Strategic Intervention

Mohadese Daemi 1 ORCID logo, Ahmad Rashki 1* ORCID logo, Saeid Salari 1 ORCID logo, Zahra Rashki Ghaleh Noo 2 ORCID logo, Sadeq Shabani 3

1 Faculty of Veterinary Medicine, Department of Physiopathology, University of Zabol, Zabol, Iran
2 Faculty of Medicine, Department of Microbiology, University of Medical Science of Zabol, Zabol, Iran
3 Department of Biological Sciences, Biomolecule Science Institute, Florida International University, Miami. FL, United States
*Corresponding Author: Ahmad Rashki, Email: ah_rashki@usal.es

Abstract

Introduction: Klebsiella pneumoniae stands as a significant opportunistic pathogen, often implicated in hospital-acquired infections. The escalating levels of antibiotic resistance, propelled by novel bacterial survival mechanisms, present a dire challenge, leading to treatment failures and amplified mortality rates. Efflux pump systems which play a pivotal role in antibiotic resistance actively expel toxic compounds, thereby fostering bacterial survival and the emergence of resilient strains. This study endeavored to examine the frequency of efflux genes in clinical isolates of K. pneumoniae.

Methods: Ninety-six non-repetitive clinical isolates of K. pneumoniae were obtained from patients attending Khatam-Al-Anbeya hospital in Zahedan, Iran. Standard biochemical laboratory methods were employed to identify all bacterial isolates. Furthermore, genomic DNA extraction was performed using the boiling method, followed by multiplex polymerase chain reaction (PCR) targeting the AcrAB, MdtK, and TolC efflux pump genes.

Results: Our findings revealed high prevalence rates: 94 (97.91%) for AcrAB, 92 (95.83%) for TolC, and 43(44.79%) for MdtK genes, indicating the widespread presence of efflux pump coding genes in K. pneumoniae isolates. Moreover, 92 isolates contained multiple studied genes.

Conclusion: Efflux pump-mediated antibiotic resistance represents a significant challenge in the treatment of K. pneumoniae infections. This study highlights the urgent need for strategies to combat efflux pump-mediated resistance and preserve the efficacy of antibiotic therapies against this clinically important pathogen.

Keywords: Klebsiella pneumoniae, Efflux genes, Clinical isolates, Antibiotic resistance

Please cite this article as follows: Daemi M, Rashki A, Salari S, Rashki Ghaleh Noo Z, Shabani S. Unveiling efflux pump-mediated antibiotic resistance in Klebsiella pneumoniae clinical isolates: a call for strategic intervention. Int J Basic Sci Med. 2024;9(1):1-5. doi:10.34172/ijbsm.46653.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 264

Your browser does not support the canvas element.


PDF Download: 56

Your browser does not support the canvas element.