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Submitted: 21 Mar 2024
Revision: 31 Mar 2024
Accepted: 06 Apr 2024
ePublished: 27 May 2024
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  Abstract View: 47

Original Article

Unveiling Efflux Pump-Mediated Antibiotic Resistance in Klebsiella pneumoniae Clinical Isolates: A Call for Strategic Intervention

Mohadeseh Daemii ORCID logo, ahmad rashki* ORCID logo, Saeed Salari ORCID logo, zahra rasheki Ghaleh Noo ORCID logo, sadeq shabani

Abstract

Introduction: Klebsiella pneumoniae (K. pneumoniae) stands as a significant opportunistic pathogen, often implicated in hospital-acquired infections. The escalating levels of antibiotic resistance, propelled by novel bacterial survival mechanisms, present a dire challenge, leading to treatment failures and amplified mortality rates. Efflux pump systems, playing a pivotal role in antibiotic resistance, actively expel toxic compounds, thereby fostering bacterial survival and the emergence of resilient strains. This study endeavours to examine the frequency of efflux genes in clinical isolates of K. pneumoniae. Methods: We obtained ninety-six non-repetitive clinical isolates of K. pneumoniae from patients attending Khatam-Al-Anbeya Hospital in Zahedan, Iran. Standard biochemical laboratory methods were employed to identify all bacterial isolates. Genomic DNA extraction was performed using the boiling method, followed by multiplex PCR targeting the AcrAB, MdtK and TolC efflux pump genes. Results: Our findings reveal high prevalence rates: 94 (97.91%) for AcrAB, 92(95.83%) for TolC, and 43(44.79%) for MdtK genes, indicating widespread presence of efflux pump coding genes in K. pneumoniae isolates. Notably, ninety and two of isolates contain multiple studied genes. Conclusion: Efflux pump-mediated antibiotic resistance represents a significant challenge in the treatment of K. pneumoniae infections. Our study highlights the urgent need for strategies to combat efflux pump-mediated resistance and preserve the efficacy of antibiotic therapies against this clinically important pathogen.
Keywords: Klebsiella pneumoniae, Efflux genes, Clinical isolates, Antibiotic resistance
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